Equipment

For protein production in prokaryotic (BL21-DE3 and its derivatives) or eukaryotic (CHO-K1, CHO-S, ExpiCHO-S, Expi293) systems, we have Infors agitators (with or without CO2), a New Brunswick bioreactor for eukaryotic cultures with single-use vessel (volumes from 5L to 14L) and a Sartorius bacterial fermenter. P2R is also equipped for acellular RTS (Roche) production.

Thanks to a “SARTOFLOW Study” system (Sartorius) with a 10L tank, we can concentrate by tangential filtration the proteins produced in large volumes of culture before their purification. P2R has several purification stations: 1 ÄKTA Purifier 10 and 1 ÄKTA FPLC from GE Healthcare, 1 NGC Quest, 1 NGC Quest Plus and 1 NGC for tandem purification from Bio-Rad, and 1 HPLC Alliance from Waters.

Thanks to the Waters Alliance system, we can carry out analytical controls by SEC or RPC with a refrigerated autosampler (120 vials). The Prometheus NT.48 system (nanotemper), equipped with an aggregation monitoring system, enables us to test the thermal stability of proteins by nano differential scanning fluorimetry. This system enables us to determine the Tm of proteins and assess their stability over time. We can test protein storage conditions: buffers, temperature.