Routine production of:
- MHC/peptide class I complexes (peptide to be provided by the user):
- Human: HLA-A2, HLA-B7, HLA-B27, HLA-B35, HLA-E
- Murine: H2Kb
- Rat: RT1Aa, RT1Af
- Human MicA
- Murine MR1 (ligand to be provided by the user)
- Custom production of MHC/peptide class I complexes: other heavy chains on request
Service, for example:
- Purification of culture supernatants
- Analytical controls of proteins or peptides
- Protein concentration by frontal filtration under nitrogen or tangential pressure (from 20 ml to 15 litres)
- Protein production in bacteria (scale mg to hundreds of mg)
- Production in mammalian cells: agitated cultures (< 3 litres) or bioreactor (< 15 litres)
- Determination and elimination of endotoxins
- Protein production in acellular system (RTS 500)
- Collaborations with users to monitor the entire project, from production steps to purification